Figure 5.

Hypotonic conditioning dissociated AQP1 interaction from band 3 in erythrocyte IOVs. RBC-IOVs colabeled with BRIC170-Alexa Fluor 488 and anti-AQP1-Alexa Fluor 568 conjugates were first suspended in HBSS and loaded into a microscope slide channel. Additional ddH₂O was then injected into the channel. When the milieu changed to HBSS containing 20% more ddH₂O, fluorescence lifetimes of Eg in the presence of acceptor Qr (Eg × Qr) were significantly higher than in HBSS. Eg × Qr interaction in Mi.III IOVs was similarly affected by hypotonic conditioning. Each group was compiled from 3 to 27 sample measurements (means ± sem). *P < 0.05, **P < 0.001; ^##P < 10⁻⁶.