Figure 3.

RvD1 biosynthesis and 5-LOX expression in ECs and VSMCs. A, B) Time- and concentration-dependent formation of RvD1 in ECs (A) or VSMCs (B) incubated with 17-HDHA as indicated. Results are shown as net RvD1 produced (pg/ml medium recovered; n = 3). *P ≤ 0.05 compared with untreated ctrl at respective time points. ^†P ≤ 0.05 compared with 4-h treatment (2-way ANOVA with Bonferroni's post hoc test). C, D) Time-dependent expression of 5-LOX in ECs (C) or VSMCs (D) treated with 17-HDHA (1 µM) as assessed by Western blot analysis. Data are means ± sem (n = 3). *P ≤ 0.05 compared with ctrl (unpaired Student’s t test).