Figure 6.

AMPK may mediate miR-184 expression at the transcriptional level. A) Pri-miR-184 expression was measured by RT-qPCR in isolated islets from βAMPKdKO (green) and littermate control (C, black) mice. B) Integration of ATAC-seq enrichment profiles with ENCODE CTCF and transcription factors ChIP-seq datasets. Sequencing tracks for the ∼100-kb genomic region upstream miR-184 show 2 enriched ATAC-seq peaks (red arrows) in βLKB1KO (green) vs. control (black) islets. CTCF ChIP-seq profiles in liver show enrichment of this factor within the most proximal ATAC-seq differential peak. Binding enrichment of other transcriptional regulators (CH12 cells) is observed at both differential ATAC-seq peaks. Alignment with the human DNA track is presented in blue.