Table 5. Detailed results of external validation.
For each strain, the identification results for the two deposits are given. %ID are confidence values given by the VITEK MS system for each identification result. COS-B = Columbia Blood Agar (bioMérieux, 43 041); BBA = Brucella blood agar (bioMérieux); COS-O = Columbia agar with 5% sheep blood (Oxoïd).
| SAMPLE INFORMATION | IDENTIFICATION RESULTS | |||||
|---|---|---|---|---|---|---|
| Strain name | Species (biovar) | Culture condition | # of peaks | Identification type | Identification | % ID |
| 75–3 | B. melitensis (1) | COS-B (48h) | 120 | Single choice | Brucella melitensis | 98.15 |
| 99 | Single choice | Brucella melitensis | 99.99 | |||
| 78–158 | B. melitensis (1) | COS-B (48h) | 125 | Single choice | Brucella melitensis | 83.06 |
| 118 | Single choice | Brucella melitensis | 99.95 | |||
| 88–44 | B. melitensis (1) | COS-B (48h) | 165 | Single choice | Brucella melitensis | 99.99 |
| 141 | Single choice | Brucella melitensis | 99.99 | |||
| 1109 | B. melitensis (1) | COS-B (48h) | 121 | Single choice | Brucella melitensis | 99.99 |
| 127 | Single choice | Brucella melitensis | 99.99 | |||
| Rev1 | B. melitensis (1) | COS-B (48h) | 134 | Single choice | Brucella melitensis | 99.8 |
| 136 | Single choice | Brucella melitensis | 99.99 | |||
| 16M+GFP | B. melitensis (1) | COS-B (48h) | 129 | Single choice | Brucella melitensis | 99.99 |
| 158 | Single choice | Brucella melitensis | 98.44 | |||
| 72–59 | B. melitensis (3) | COS-B (48h) | 150 | Single choice | Brucella melitensis | 98.1 |
| 129 | Single choice | Brucella melitensis | 99.76 | |||
| 77–47 | B. melitensis (3) | COS-B (48h) | 127 | Single choice | Brucella melitensis | 99.99 |
| 124 | Single choice | Brucella melitensis | 99.99 | |||
| 78–13 | B. melitensis (3) | COS-B (48h) | 111 | Single choice | Brucella melitensis | 63.65 |
| 102 | Single choice | Brucella melitensis | 99.64 | |||
| 81–44 | B. melitensis (3) | COS-B (48h) | 122 | No identification | ||
| 101 | Single choice | Brucella melitensis | 99.51 | |||
| 81–140 | B. melitensis (3) | COS-B (48h) | 148 | Single choice | Brucella melitensis | 99.99 |
| 147 | Single choice | Brucella melitensis | 99.96 | |||
| 82–73 | B. melitensis (3) | COS-B (48h) | 99 | Single choice | Brucella melitensis | 87.07 |
| 101 | Single choice | Brucella melitensis | 99.97 | |||
| 82–87 | B. melitensis (3) | COS-B (48h) | 121 | Single choice | Brucella melitensis | 99.79 |
| 123 | Single choice | Brucella melitensis | 89.74 | |||
| 90–129 | B. melitensis (3) | COS-B (48h) | 110 | Single choice | Brucella melitensis | 99.99 |
| 125 | Single choice | Brucella melitensis | 99.99 | |||
| 91–244 | B. melitensis (3) | COS-B (48h) | 91 | No identification | ||
| 101 | Single choice | Brucella melitensis | 99.99 | |||
| 79–185 | B. melitensis (3) | COS-B (48h) | 163 | Single choice | Brucella melitensis | 89.68 |
| 162 | No identification | |||||
| 2308 | B. abortus (1) | COS-B (48h) | 165 | Single choice | Brucella abortus | 99.99 |
| 154 | Single choice | Brucella abortus | 99.99 | |||
| S19 | B. abortus (1) | BBA (72h) | 66 | Low Discrimination | Brucella abortus | 50.14 |
| 80 | Single choice | Brucella abortus | 99.99 | |||
| RB51 | B. abortus (1) | BBA (96h) | 173 | No identification | ||
| 104 | No identification | |||||
| 97-4775-11 | B. abortus (1) | COS-B (48h) | 134 | Single choice | Brucella abortus | 99.97 |
| 136 | Single choice | Brucella abortus | 81.79 | |||
| 75–17 | B. abortus (3) | COS-B (48h) | 127 | Single choice | Brucella abortus | 99.99 |
| 125 | Single choice | Brucella abortus | 99.99 | |||
| 83–227 | B. abortus (3) | COS-B (48h) | 137 | Single choice | Brucella abortus | 98.34 |
| 101 | Single choice | Brucella abortus | 99.87 | |||
| 83–233 | B. abortus (3) | COS-B (48h) | 136 | Single choice | Brucella abortus | 99.99 |
| 117 | Single choice | Brucella abortus | 99.99 | |||
| 79–153 | B. abortus (3) | COS-B (48h) | 130 | Single choice | Brucella abortus | 99.99 |
| 136 | Single choice | Brucella abortus | 99.99 | |||
| 82–41 | B. abortus (3) | COS-B (48h) | 130 | Single choice | Brucella abortus | 99.99 |
| 138 | Single choice | Brucella abortus | 99.99 | |||
| 99-9971-135 | B. abortus (7) | COS-B (72h) | 81 | Single choice | Brucella abortus | 99.99 |
| 105 | Single choice | Brucella abortus | 99.99 | |||
| 03-4923-239-D | B. abortus (7) | COS-B (48h) | 148 | Single choice | Brucella abortus | 99.99 |
| 149 | Single choice | Brucella abortus | 99.99 | |||
| 77–9 | B. abortus (9) | COS-B (48h) | 94 | Single choice | Brucella abortus | 99.99 |
| 118 | Single choice | Brucella abortus | 99.99 | |||
| 80–133 | B. abortus (9) | COS-B (48h) | 98 | Single choice | Brucella abortus | 99.99 |
| 115 | Single choice | Brucella abortus | 87.95 | |||
| 04-1361Sisak-4 | B. suis (1) | COS-O (72h) | 87 | Single choice | Brucella suis | 99.99 |
| 78 | Single choice | Brucella suis | 99.99 | |||
| Mex 51 | B. canis | COS-B (48h) | 140 | Single choice | Brucella suis | 99.98 |
| 158 | No identification | |||||
| 76250 | B. ovis | COS-B + 5% CO2 (96h) | 125 | No identification | ||
| 107 | No identification | |||||
| 91268 | B. ovis | COS-B + 5% CO2 (96h) | 116 | No identification | ||
| 104 | Single choice | Brucella ovis | 99.99 | |||
| 91212 | B. ovis | COS-B + 5% CO2 (96h) | 130 | Single choice | Brucella ovis | 99.99 |
| 101 | Single choice | Brucella ovis | 99.96 | |||
| F5/99 | B. ceti | COS-B + 5% CO2 (96h) | 106 | No identification | ||
| 91 | No identification | |||||
| UK3/05 | B. ceti | COS-B + 5% CO2 (96h) | 127 | No identification | ||
| 121 | Single choice | Brucella ceti/pinnipedialis | 99.98 | |||
| UK9/99 | B. pinnipedialis | COS-B + 5% CO2 (96h) | 118 | Single choice | Brucella ceti/pinnipedialis | 83.28 |
| 126 | Single choice | Brucella ceti/pinnipedialis | 99.99 | |||
| 36/94 | B. ceti | COS-B + 5% CO2 (96h) | 112 | No identification | ||
| 119 | No identification | |||||
| 49/94 | Brucella sp. (B. ceti ?) | COS-B + 5% CO2 (96h) | 119 | Single choice | Brucella ceti/pinnipedialis | 99.99 |
| 128 | Single choice | Brucella ceti/pinnipedialis | 99.96 | |||
| 02/611 | Brucella sp. (B. ceti-like) | COS-B + 5% CO2 (96h) | 148 | Single choice | Brucella ceti/pinnipedialis | 99.99 |
| 133 | Single choice | Brucella ceti/pinnipedialis | 100 | |||
| 15/95 | Brucella sp. (B. pinnipedialis ?) | COS-B + 5% CO2 (96h) | 112 | No identification | ||
| 114 | No identification | |||||
| 56/94 | B. pinnipedialis | COS-B + 5% CO2 (96h) | 116 | No identification | ||
| 126 | Single choice | Brucella ceti/pinnipedialis | 99.77 | |||
| 96/408 | B. pinnipedialis | COS-B + 5% CO2 (96h) | 131 | Single choice | Brucella ceti/pinnipedialis | 99.99 |
| 97 | No identification | |||||
| CCM4915 | B. microti | COS-B (24h) | 162 | No identification | ||
| 181 | No identification | |||||
| 5K33 | B. neotomae | COS-B (48h) | 145 | No identification | ||
| 127 | No identification | |||||
| NF2637 | Brucella sp. | COS-B (24h) | 114 | Single choice | Brucella inopinata | 99.99 |
| 144 | Single choice | Brucella inopinata | 77.86 | |||
| NF2653 | Brucella sp. | COS-B (24h) | 141 | No identification | ||
| 157 | No identification | |||||
| B13-0095 | Brucella sp. | COS-B (24h) | 136 | Single choice | Brucella inopinata | 100 |
| 129 | Single choice | Brucella inopinata | 99.99 | |||
| ATCC48188 | Ochrobactrum anthropi | COS-B (48h) | 127 | Single choice | Ochrobactrum anthropi | 100 |
| 113 | No identification | |||||
| LMG3301 | Ochrobactrum intermedium | COS-B (48h) | 122 | Single choice | Ochrobactrum intermedium | 100 |
| 118 | Single choice | Ochrobactrum intermedium | 100 | |||