Fig. 4 |. HSD increases inhibitory eNOS phosphorylation.
a, HSD (8 weeks) increases the inhibitory phosphorylation of eNOS at Thr495 in isolated pial microvascular preparations (⋆P = 0.0051 vs. ND; microvessels isolated from 7 ND and 9 HSD mice per group; unpaired t test, two-tailed). b, The activatory phosphorylation of eNOS at Ser1177 is not affected by HSD (P = 0.6615 vs. ND; microvessels isolated from 8 ND and 7 HSD mice per group; unpaired t test, two-tailed). Data are derived from 2 independent experiments and expressed as mean ± s.e.m. Immunoblots in a and b are cropped; full gel pictures for immunoblots are shown in Supplementary Fig. 12.