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Clonogenic assay of nanoparticles or free DOX or ELC in HepG2 cells.
Notes: (A) Representative images of single-cell clone proliferation, stained with crystal violet. (B) Quantification of the results (A). Data are presented as mean±standard deviation (n=3). *P<0.05; **P<0.01; ***P<0.001.
Abbreviations: DOX, doxorubicin; ELC, elacridar; NB, blank nanoparticle; ND, DOX-loaded nanoparticle; NDE, nanoparticles loaded with DOX and ELC at the optimized ratio; NE, ELC-loaded nanoparticle.
