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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Mol Microbiol. 2018 Oct 12;110(3):469–483. doi: 10.1111/mmi.14113

Figure 4. The queC deletion strain show deficiency in Q modification and dADG-dependent modification and increased transformation efficiency with unmodified DNA.

Figure 4.

(A) Q− phenotype in the queC deletion strain can be complemented by preQ0 feeding. Q incorporation into tRNAAspGUC tRNA was detected in total tRNA extracted at 0, 20, 40 and 60 min after supplementing S. Montevideo culture medium with 5 nM preQ0 or DMSO in M9 broth containing 0.4% arabinose. tRNAASPGUC was visualized by hybridization with a specific biotinylated probe. tRNAs modified with Q (upper bands) migrate slower than the unmodified tRNA (lower bands) (Igloi & Kossel, 1985). (B) pUC19 DNA (10 ng) extracted from different S. Montevideo mutant donor strains was transformed in the WT host and YYF3022. (C) Modified and unmodified pUC19 DNA (10 ng) was transformed in different S. Montevideo mutant host cells. The average of three experiments is shown, with error bars representing SD (***P < 0.001, two-tailed Student’s t-test).