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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: J Invest Dermatol. 2018 Jul 14;138(11):2432–2442. doi: 10.1016/j.jid.2018.04.029

Figure 4. Fibronectin adhesion to integrin α5β1 induces FAK phosphorylation.

Figure 4.

(a) Phospho-FAK immunostainings of UW_BCC_T2 and ASZ_001 on poly-L-lysine or fibronectin.

(b) Phospho-FAK immunostainings of UW_BCC_T2 and ASZ_001 on fibronectin treated with DMSO or K34C.

(c) Phospho-FAK immunostainings of UW_BCC_T2 on fibronectin treated with isotype (control) or P1D6 antibodies.

(d) Quantification of membranous phospho-FAK shown in (b) and (c).

(e) Western blots for phospho(Y397)- and total-FAK in UW_BCC_T2 and ASZ_001 on fibronectin treated with DMSO or K34C, and UW_BCC_T2 on fibronectin treated with isotype (control) or P1D6 antibodies.

(f) Phospho-FAK stainings and quantification in nodular- compared to infiltrative-like areas of UW_BCC_T2 tumors injected with TGFβ. Scale bars indicate 100μm.

White arrowheads indicate membranous phospho-FAK. Scale bars indicate 20μm (unless specified). Columns and error bars represent the mean ± SD for n ≥ 3 per group. *p < 0.05, **p < 0.01 and ***p < 0.001.