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. 2018 Feb 12;15(10):875–887. doi: 10.1038/cmi.2017.150

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© The Chinese Society of Immunology and The University of Science and Technology of China, All rights reserved 2017

Open Access This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if thematerial is not included under the CreativeCommons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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3DISCO clearing and light sheet microscopy (LiSM) imaging of whole lung lobes. (a) Clearing of separated lung lobes using organic solvents. Separated lung lobes (left) were dehydrated in increasing concentrations of THF followed by optical clearing in DBE (right) before LiSM imaging. (b) Diagrammatic representation of LiSM set-up showing sample orientation, excitation by a thin light sheet and image acquisition. (c, d) Representative LiSM micrographs from 3DISCO-cleared lung lobes, showing sectional and 3D views of an imaged lobe from a wild type (WT)mouse. AF, autofluorescence.