Figure 11.

Cell-to-cell contact assay. Proliferation of autologous EC treated with CFSE was analyzed by flow cytometry when either co-cultured or cultured in a transwell plate with mock- or HIV-1_NL4-3-B cells (ratio Breg: EC of 2:1) at day 3 post co-culture. EC-CFSE were directly co-cultured or cultured in a transwell system with mock-treated or HIV_NL4-3-treated B cells. The percentage of relative proliferation was calculated as follows: (% of CD4+ or CD8+ T cell proliferation in HIV-1_NL4-3-treated condition*100 / % of CD4+ or CD8+ T cell proliferation in mock-treated condition) in co-culture or in transwell assay. Average±s.e.m. of five experiments for each condition is shown. *P<0.05 when comparing Co-culture versus Transwell conditions.