Table 1.

Impact of axotomy on major classes of sacral preganglionic neurons.

Neuronal class by marker(s)	% All preganglionic neurons	Expression of marker after axotomy	Expression of ATF3 in neuron class after axotomy
SOM ± NK1R	30.4 ± 2.2% SOM	Decrease in SOM	50.6 ± 3.0% SOM neurons
	33.0 ± 1.3% SOM	Decrease in NK1Ra	53.1 ± 7.2% NK1R neuronsa
CAL ± NK1R	26.0 ± 1.9% CAL	No change in CAL	33.1 ± 2.6% CAL neurons
	24.9 ± 1.5% CAL	Decrease in NK1Ra	53.1 ± 7.2% NK1R neuronsa
Hsp25	21.8 ± 2.5%b	Increase	76.4 ± 2.3%

Neurons expressing both SOM and CAL were scarce so for summary purposes they are shown as separate classes of neurons. The proportion of preganglionic neurons expressing SOM or CAL was determined in two sets of NK1R coexpression experiments, so both results are shown here in column 2.

^aIt is not known if the axotomy-induced decrease in NK1R-immunoreactivity and upregulation of ATF3 in this neuron class occurs in SOM, CAL, or both NK1R-expressing classes, so the data have been repeated in each row. The potential coexpression of SOM, NK1R, and CAL by Hsp25 neurons was not investigated.

^bHsp25-immunoreactivity was only visible after axotomy.