Table 2.
Summary table of Vibrio phage BONAISHI predicted proteins that contained relevant annotation information as determined from significant BLASTP hits (e-value < e-3) against the GenBank non-redundant and CAZY databases.
| Predicted function | START | STOP | Size (n) | Best-hit | Classification | E-value | Score (bits) | N°accession | |
|---|---|---|---|---|---|---|---|---|---|
| ORF01c | hypothetical protein | 653 | 1753 | 1100 | Pseudocercospora musae | Fungi | 2.00E-07 | 66.00 | KNG49823.1 |
| ORF02c | SbcC-like protein | 1750 | 2184 | 435 | Pseudomonas phage 201phi2-1 | Myoviridae | 1.00E-17 | 88.00 | YP_001956973 |
| ORF03 | conserved hypothetical protein | 2241 | 3119 | 879 | Pseudomonas phage Phikz | Myoviridae | 2.00E-32 | 130.00 | NP_803730 |
| ORF05 | PaaR repeat-containing protein | 3722 | 4018 | 297 | Vibrio cholerae | ⋎-proteobacteria | 3.00E-21 | 89.00 | WP_057643503 |
| ORF06c | conserved hypothetical protein | 4055 | 4657 | 603 | Pseudomonas phage Phikz | Myoviridae | 1.00E-25 | 203.00 | AAL83062 |
| ORF07c | virion structural protein | 4667 | 5962 | 1296 | Pseudomonas phage KTN4 | Myoviridae | 7.00E-32 | 147.00 | ANM44952.1 |
| ORF10 | ribonuclease H | 6889 | 8271 | 1383 | Pseudomonas phage 201phi2-1 | Myoviridae | 3.00E-25 | 117.00 | YP_001956963 |
| ORF12 | uvsx protein | 9269 | 10744 | 1476 | Pseudomonas phage 201phi2-1 | Myoviridae | 1.00E-89 | 290.00 | YP_001956960 |
| ORF13 | conserved hypothetical protein | 10746 | 11108 | 363 | Pseudomonas phage phiPA3 | Myoviridae | 3.00E-28 | 108.00 | AEH03597 |
| ORF15 | virion structural protein | 11460 | 12125 | 666 | Pseudomonas phage phiPA3 | Myoviridae | 2.00E-27 | 113.00 | AEH03595 |
| ORF17 | hypothetical protein | 12558 | 13256 | 699 | Erwinia phage Ea35-70 | Myoviridae | 8.00E-09 | 64.00 | YP_009004948 |
| ORF22c | conserved hypothetical protein | 16270 | 18384 | 2115 | Ralstonia phage RSL2 | Myoviridae | 3.00E-74 | 261.00 | BAQ02568 |
| ORF23c | glycoside hydrolase | 18393 | 24110 | 5718 | Ralstonia phage RP31 | Myoviridae | 2.00E-70 | 277.00 | BAW19303.1 |
| ORF24 | RNA polymerase beta prime subunit | 24183 | 25850 | 1668 | Pseudomonas phage OBP | Myoviridae | 1.00E-110 | 346.00 | YP_004958184 |
| ORF25 | RNA polymerase beta subunit | 25847 | 28741 | 2895 | Ralstonia phage RSF1 | Myoviridae | 1.00E-102 | 358.00 | BAS04832 |
| ORF26 | RNA polymerase beta subunit | 29242 | 30369 | 1128 | Pseudomonas phage 201phi2-1 | Myoviridae | 2.00E-61 | 223.00 | YP_001956996 |
| ORF29 | conserved hypothetical protein | 32135 | 32860 | 726 | Pseudomonas phage Noxifer | Myoviridae | 3.00E-21 | 110.00 | ARV77361.1 |
| ORF30 | virion structural protein | 32871 | 33641 | 771 | Ralstonia phage RSF1 | Myoviridae | 3.00E-31 | 124.00 | BAS04828 |
| ORF36c | tail tube protein | 36168 | 37040 | 873 | Pseudomonas phage 201phi2-1 | Myoviridae | 2.00E-24 | 108.00 | YP_001956757 |
| ORF37c | tail shealth protein | 37089 | 39137 | 2049 | Erwinia phage Ea35-70 | Myoviridae | 2.00E-83 | 286.00 | YP_009004971 |
| ORF38 | hypothetical protein | 39208 | 40278 | 1071 | Erwinia phage Ea35-70 | Myoviridae | 3.00E-09 | 67.00 | YP_009004972 |
| ORF39 | virion structural protein | 40288 | 42819 | 2532 | Pseudomonas phage 201 phi2-1 | Myoviridae | 2.00E-55 | 226.00 | YP_001956754.1 |
| ORF40 | virion structural protein | 42831 | 44483 | 1653 | Pseudomonas phage 201phi2-1 | Myoviridae | 3.00E-27 | 125.00 | YP_001956753 |
| ORF41 | terminase large subunit | 44535 | 46691 | 2157 | Pseudomonas phage 201phi2-1 | Myoviridae | 1.00E-142 | 442.00 | YP_001956731 |
| ORF43c | conserved hypothetical protein | 47798 | 49027 | 1230 | Pseudomonas phage Noxifer | Myoviridae | 4.00E-37 | 164.00 | ARV77197.1 |
| ORF44 | HD domain protein | 49121 | 49720 | 600 | Salmonella phage SPN3US | Myoviridae | 3.00E-23 | 101.00 | AEP84084 |
| ORF49c | RNA-binding protein | 52606 | 54225 | 1620 | Actinomyces massiliensis | Actinobacteria | 2.00E-73 | 251.00 | WP_017194325 |
| ORF58c | conserved hypothetical protein | 58317 | 59075 | 759 | Erwinia phage Ea35-70 | Myoviridae | 1.00E-22 | 102.00 | YP_009005001 |
| ORF59 | tubulin-like protein | 59178 | 60167 | 990 | Erwinia phage Ea35-70 | Myoviridae | 3.00E-21 | 101.00 | YP_009005002 |
| ORF68 | hypothetical protein | 64017 | 64424 | 408 | Ralstonia phage RSL2 | Myoviridae | 7.00E-06 | 52.00 | BAQ02532 |
| ORF69 | conserved hypothetical protein | 64596 | 66782 | 2187 | Erwinia phage Ea35-70 | Myoviridae | 1.00E-105 | 345.00 | YP_009005012 |
| ORF70c | hypothetical protein | 66814 | 67875 | 1062 | Ralstonia phage RSF1 | Myoviridae | 9.00E-08 | 63.00 | BAS05022 |
| ORF71 | hypothetical protein | 68148 | 70115 | 1967 | ⋎-protebacteria bacterium | ⋎-proteobacteria | 1.00E-15 | 94.00 | OUV32520.1 |
| ORF72 | RNA polymerase beta prime subunit | 70204 | 71697 | 1494 | Erwinia phage Ea35-70 | Myoviridae | 3.00E-44 | 171.00 | YP_009005015 |
| ORF73 | conserved hypothetical protein | 71795 | 72433 | 639 | Erwinia phage Ea35-70 | Myoviridae | 9.00E-15 | 79.00 | YP_009005021 |
| ORF76 | nuclease SbcC subunit | 73326 | 74498 | 1173 | Pseudomonas phage PhipA3 | Myoviridae | 1.00E-46 | 172.00 | AEH03486 |
| ORF77 | conserved hypothetical protein | 74495 | 75289 | 795 | Erwinia phage PhiEaH1 | Siphoviridae | 9.00E-23 | 103.00 | YP_009010069 |
| ORF78 | hypothetical protein | 75301 | 75972 | 672 | Uncultured bacterium | Bacteria | 2.00E-06 | 56.00 | EKD22589 |
| ORF79 | hypothetical protein | 76106 | 77656 | 1551 | Pseudomonas phage phiPA3 | Myoviridae | 9.00E-06 | 59.00 | AEH03489 |
| ORF80 | conserved hypothetical protein | 77691 | 79211 | 1521 | Pseudomonas phage phiPA3 | Myoviridae | 1.00E-16 | 93.00 | AEH03490 |
| ORF81 | hypothetical protein | 79251 | 81056 | 1806 | Gossypium arboreum | Magnoliopsida | 1.00E-06 | 63.00 | KHG21929 |
| ORF82c | hypothetical protein | 81109 | 81456 | 348 | Pseudomonas phage PA7 | Myoviridae | 5.00E-09 | 59.00 | AFO71119 |
| ORF83 | RNA polymerase beta subunit | 81518 | 83584 | 2067 | Pseudomonas phage Phabio | Myoviridae | 8.00E-66 | 260.00 | ARV76743.1 |
| ORF84 | RNA polymerase beta prime subunit | 83584 | 85557 | 1974 | Ralstonia phage RSF1 | Myoviridae | 1.00E-50 | 196.00 | BAS05006 |
| ORF85 | helicase | 85657 | 87198 | 1542 | Pseudomonas phage OBP | Myoviridae | 1.00E-40 | 176.00 | AEV89521.1 |
| ORF86 | Clp protease subunit | 87276 | 87848 | 573 | Bacillus cereus | Bacilli | 5.00E-05 | 52.00 | WP_048520069 |
| ORF87 | ATP-dependent Clp protease proteolytic subunit | 87848 | 88339 | 492 | Dactylosporangium aurantiacum | Actinobacteria | 1.00E-27 | 110.00 | WP_033356707 |
| ORF89c | conserved hypothetical protein | 88833 | 90389 | 1557 | Pseudomonas phage PA7 | Myoviridae | 2.00E-15 | 89.00 | AFO71110 |
| ORF92 | RNA polymerase beta prime subunit | 91656 | 92915 | 1260 | Ralstonia phage RSF1 | Myoviridae | 8.00E-49 | 180.00 | BAS04991 |
| ORF96c | DNA polymerase polB | 96090 | 97820 | 1731 | Ralstonia phape RP12 | Unclassified virus | 6.00E-107 | 343.00 | BAW19225.1 |
| ORF97 | virion structural protein | 97895 | 99190 | 1296 | Erwinia phage PhiEaH1 | Siphoviridae | 2.00E-23 | 111.00 | YP_009010288 |
| ORF99c | virion structural protein | 101147 | 104005 | 2859 | Pseudomonas phage 201phi2-1 | Myoviridae | 1.00E-93 | 327.00 | YP_001956873 |
| ORF100c | virion structural protein | 104007 | 105122 | 1116 | Ralstonia phage RSL2 | Myoviridae | 2.00E-49 | 180.00 | BAQ02702 |
| ORF101 | capsid protein* | 105166 | 106242 | 1077 | Ralstonia phage RSF1 | Myoviridae | 3.00E-22 | 105.00 | BAS04975 |
| ORF102 | virion structural protein | 106257 | 107141 | 885 | Ralstonia phage RSF1 | Myoviridae | 4.00E-07 | 60.00 | BAS04974 |
| ORF104 | hypothetical protein | 107713 | 109107 | 1394 | ⋎-protebacteria bacterium | ⋎-proteobacteria | 1.00E-12 | 83.00 | OUV32343.1 |
| ORF107 | conserved hypothetical protein | 111644 | 113257 | 1614 | Ralstonia phage RSF1 | Myoviridae | 8.00E-22 | 108.00 | BAS04969 |
| ORF108 | virion structural protein | 113257 | 114459 | 1203 | Pseudomonas phage PhiPA3 | Myoviridae | 8.00E-12 | 77.00 | AEH03528 |
| ORF110 | virion structural protein | 115149 | 116531 | 1383 | Pseudomonas phage PhiPA3 | Myoviridae | 2.00E-28 | 126.00 | AEH03530 |
| ORF111c | helicase | 116571 | 118181 | 1611 | Pseudomonas phage 201phi2-1 | Myoviridae | 2.00E-48 | 1884.00 | YP_001956921 |
| ORF113 | major capsid protein | 118843 | 121035 | 2193 | Erwinia phage Ea35-70 | Myoviridae | 2.00E-23 | 116.00 | YP_009005109 |
| ORF115 | conserved hypothetical protein | 122460 | 124031 | 1572 | Ralstonia phage RSL2 | Myoviridae | 1.00E-46 | 179.00 | BAQ02643 |
| ORF118c | holliday-junction resolvase | 127427 | 127987 | 561 | Pseudomonas phage Phabio | Myoviridae | 4.00E-18 | 99.00 | ARV76843.1 |
| ORF119c | virion structural protein | 128029 | 128865 | 837 | Pseudomonas phage 201phi2-1 | Myoviridae | 8.00E-44 | 159.00 | YP_001956947 |
| ORF120c | virion structural protein | 128878 | 130947 | 2070 | Pseudomonas phage phiPA3 | Myoviridae | 7.00E-72 | 256.00 | AEH03570 |
| ORF121 | virion structural protein | 131049 | 133649 | 2601 | Pseudomonas phage phabio | Myoviridae | 4.00E-61 | 245.00 | ARV76832.1 |
| ORF133 | hypothetical protein | 139060 | 140100 | 1041 | Vibrio tasmaniensis | ⋎-proteobacteria | 2.00E-11 | 73.00 | WP_017112059 |
| ORF138 | glycoside hydrolase | 141805 | 142716 | 912 | Aureimonas altamirensis | α-proteobacteria | 2.00E-49 | 174.00 | BAT26087 |
| ORF141 | hypothetical protein | 145070 | 148201 | 3132 | Psychromonas ingrahamii | ⋎-proteobacteria | 1.00E-08 | 72.00 | WP_011768462.1 |
| ORF143 | hypothetical protein | 150013 | 151644 | 1631 | Vibrio phage s4-7 | Unclassified virus | 2.00E-06 | 63.00 | AOQ26845.1 |
| ORF151 | hypothetical protein | 160192 | 161433 | 1242 | Colwellia phage 9A | Siphoviridae | 4.00E-08 | 66.00 | YP_006489231 |
| ORF159 | hypothetical protein | 168857 | 169801 | 945 | Escherichia phage phAPEC8 | Myoviridae | 4.00E-10 | 68.00 | YP_007348452 |
| ORF163 | hypothetical protein | 172164 | 172847 | 684 | Ruegeria halocynthiae | α-proteobactérie | 1.00E-19 | 91.00 | WP_037312174 |
| ORF167 | dead-like helicase | 176707 | 178806 | 2100 | Erwinia phage Ea35-70 | Myoviridae | 1.00E-114 | 367.00 | YP_009004923 |
| ORF172 | nicotinamide-nucleotide adenylyltransferase | 182827 | 184476 | 1650 | Vibrio phage 11895-B1 | Myoviridae | 1.00E-106 | 331.00 | YP_007673553 |
| ORF176 | nicotinamide-nucleotide adenylyltransferase | 185996 | 187114 | 1119 | Thiorhodococcus drewsii | ⋎-proteobacteria | 3.00E-46 | 170.00 | WP_007039048 |
| ORF177 | nicotinamide phosphoribosyltransferase | 187169 | 188668 | 1500 | Vibrio nigripulchritudo | ⋎-proteobacteria | 3.00E-59 | 210.00 | WP_022562194 |
| ORF181 | nicotinamide riboside transporter | 190786 | 191502 | 717 | Vibrio phage 11895-B1 | Myoviridae | 3.00E-76 | 238.00 | YP_007673552 |
| ORF189 | hypothetical protein | 196049 | 196543 | 717 | Kaistia granuli | α-proteobacteria | 1.00E-08 | 57.00 | WP_018183972 |
| ORF198 | NAD-dependent DNA ligase | 204242 | 206257 | 2016 | Vibrio maritimus | ⋎-proteobacteria | 0.00E+00 | 608.00 | WP_042496716 |
| ORF200 | phosphatase | 206698 | 207330 | 633 | Vibrio phage VH7D | Myoviridae | 6.00E-19 | 89.00 | YP_009006310 |
| ORF206 | hypothetical protein | 210803 | 211318 | 516 | Psychromonas aquimarina | ⋎-proteobacteria | 7.00E-34 | 126.00 | WP_028862581 |
| ORF209 | hypothetical protein | 213895 | 214209 | 315 | Enterovibrio calviensis | ⋎-proteobacteria | 6.00E-07 | 52.00 | WP_017007757 |
| ORF212 | transcriptional regulator | 215240 | 215764 | 525 | Leptolyngbya sp. PCC 7375 | cyanobacteria | 1.00E-05 | 53.00 | EKV01169 |
| ORF220 | hypothetical protein | 220875 | 221303 | 429 | Shewanella sp. phage 1/4 | Myoviridae | 6.00E-11 | 64.00 | YP_009100318 |
| ORF227 | hypothetical protein | 224066 | 224998 | 933 | Pseudomonas phage PhiPA3 | Myoviridae | 9.00E-06 | 57.00 | AEH03433 |
| ORF229 | hypothetical protein | 226086 | 227921 | 1835 | Vibrio phage RYC | Unclassified virus | 7.00E-70 | 243.00 | BAV81012.1 |
| ORF231 | thymidylate kinase | 228796 | 229458 | 663 | Desulfosporosinus acidiphilus | Clostridia | 8.00E-42 | 150.00 | WP_014828008 |
| ORF232 | ribonucleotide-diphosphate reductase subunit alpha | 229535 | 230446 | 912 | Aeromonas molluscorum 848 | ⋎-proteobacteria | 1.00E-132 | 385.00 | EOD53957 |
| ORF233 | ribonucleotide-diphosphate reductase subunit alpha | 230844 | 232277 | 1434 | Neisseria meningitidis | β-proteobacteria | 0.00E+00 | 635.00 | WP_049227356 |
| ORF234 | ribonucleotide-diphosphate reductase subunit beta | 232355 | 233062 | 708 | Thiomicrospira sp. Kp2 | ⋎-proteobacteria | 3.00E-96 | 294.00 | WP_040727751 |
| ORF235 | HNH endonuclease | 233205 | 233927 | 723 | Bacillus pumilus | Bacilli | 7.00E-32 | 123.00 | WP_051149989 |
| ORF236 | ribonucleotide-diphosphate reductase subunit beta | 234231 | 234617 | 387 | Shigella sonnei | ⋎-proteobacteria | 2.00E-36 | 129.00 | CSE34793 |
| ORF238 | hypothetical protein | 235508 | 237232 | 1725 | Polyangium brachysporum | β-proteobacteria | 2.00E-23 | 114.00 | WP_047195109 |
| ORF249c | hypothetical protein | 242652 | 244886 | 2235 | Vibrio mimicus | ⋎-proteobacteria | 4.00E-08 | 67.00 | WP_001015571 |
| ORF253 | phosphatase | 246359 | 247015 | 657 | Verrucomicrobium spinosum | verucomicrobia | 2.00E-22 | 97.00 | WP_009962909 |
| ORF254 | hypothetical protein | 247012 | 247842 | 831 | Shewanella sp. | ⋎-proteobacteria | 6.00E-06 | 57.00 | WP101034114.1 |
| ORF255 | ATP-binding protein | 247870 | 248553 | 684 | Vibrio phage RYC | Unclassified virus | 1.00E-36 | 137.00 | BAV81012.1 |
| ORF256c | T5 A1-like protein | 248608 | 250479 | 1872 | Caulobacter phage phiCbK | Siphoviridae | 3.00E-83 | 281.00 | YP_006988022 |
| ORF257c | hypothetical protein | 250484 | 250927 | 444 | Pseudoalteromonas (multispecies) | ⋎-proteobacteria | 9.00E-19 | 86.00 | WP_024591352 |
| ORF258 | conserved hypothetical protein | 251087 | 252592 | 1506 | Campylobacter phage CP30A | Myoviridae | 3.00E-20 | 102.00 | YP_006908082 |
| ORF259 | conserved hypothetical protein | 252655 | 253968 | 1314 | Campylobacter phage CP30A | Myoviridae | 2.00E-18 | 96.00 | YP_006908082 |
| ORF261 | phosphate starvation protein PhoH | 254632 | 255627 | 996 | Corynebacterium glucuronolyticum | Actinobacteria | 6.00E-49 | 175.00 | WP_005389286 |
| ORF269 | thymidylate synthase-complementing protein | 260653 | 262095 | 1443 | Parcubacteria | Parcubacteria | 5.00E-62 | 217.00 | KKR42866 |
| ORF272 | conserved hypothetical protein | 263205 | 263723 | 519 | Cronobacter phage vB_CsaM_GAP32 | Myoviridae | 9.00E-15 | 77.00 | YP_006987447 |
| ORF289 | conserved hypothetical protein | 273177 | 273917 | 740 | Vibrio phage vB_VhaS-a | Unclassified virus | 4.00E-18 | 100.00 | ANO57550.1 |
| ORF290 | conserved hypothetical protein | 273997 | 274665 | 668 | Vibrio phage vB_VhaS-a | Unclassified virus | 1.00E-14 | 88.00 | ANO57549.1 |
| ORF295c | hypothetical protein | 276639 | 277370 | 731 | Ralstonia phage RP12 | Myoviridae | 9.00E-10 | 73.00 | BAW19047.1 |
| ORF300 | hypothetical tail protein | 286648 | 287631 | 983 | Pseudomonas phage Phabio | Myoviridae | 7.00E-15 | 90.00 | ARV76834.1 |
| ORF301 | virion structural protein | 287726 | 288967 | 1241 | Pseudomonas phage Noxifer | Myoviridae | 3.00E-07 | 65.00 | ARV77324.1 |