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. 2018 Oct 24;9:2516. doi: 10.3389/fmicb.2018.02516

Figure 4.

Figure 4

Lack of β-hemolysin production in MRSA-ST398 harboring IEC. (A–C) CAMP test results for Streptococcus agalactiae (grown as central diameter on each plate) together with 18 MRSA-ST398-t011 isolates of equine origin. grown as central diameter on each plate) together with 18 MRSA-ST398-t011 isolates of equine origin. (A) White arrow: large hemolytic zone irrespectively of CAMP factor presence in 1–8, 10, 11, 13, 14, and 17. These isolates harbor a β-hemolysin disrupting phage carrying IEC (numbers as indicated in Table 2). (B) White arrow: enlarged hemolysis zone induced by synergistic activity of CAMP factor- and β-hemolysin production in 9, 12, 15, 16, and 18. (C) White arrow: β-hemolysin production inhibits lysis by α-hemolysin (Traber et al., 2008), resulting in weaker total hemolysis zones for the IEC-negative isolates 9, 12, 15, 16, and 18. +, positive control S. aureus ATCC 25923.