Fig. 6.

Crystal structure of the NDM-1 K224R/G232A/N233Q mutant. a Left panel, alignment of structure of wild-type NDM-1 (tan, PDB ID: 3SPU¹⁸) with that of NDM-1 K224R/G232A/N233Q (dark cyan). The zinc ions are represented as orange and gray spheres in the wild-type and mutant NDM-1 structures, respectively. The water molecule between Zn1 and Zn2 is shown as a red sphere. The side chains of residues 232–237 are shown within the boxed region of each structure. The box indicates the region of the structure shown in detail in panel B. The N- and C-termini are labeled. b Boxed region from panel (a) showing alignment of the structure of wild-type NDM-1 (tan, PDB ID: 3SPU) with that of NDM-1 K224R/G232A/N233Q (dark cyan) for residues 231 to 237. A large change in the conformation of the 231–237 region of the NDM-1 K224R/G232A/N233Q mutant is seen versus the structure of wild-type NDM-1