Fig. 7.

Structural alignments of the NDM-1 triple mutant with NDM-1/product complexes. a Structure of wild-type NDM-1 (tan) in complex with hydrolyzed ampicillin (3Q6X). Hydrolyzed ampicillin is shown in gray. Hydrogen bonds are indicated by thin black lines. b Structural alignment of wild-type NDM-1 (tan) with hydrolyzed ampicillin (gray) and the NDM-1 triple mutant (dark cyan). Hydrogen bonds are not shown for clarity. c Structural alignment from panel (b) with the wild-type NDM-1 structure removed but leaving the hydrolyzed ampicillin from the wild-type structure in place. d Structure of wild-type NDM-1 (tan) in complex with hydrolyzed imipenem (5YPI⁷). Hydrolyzed imipenem is shown in gray. e Structural alignment of wild-type NDM-1 (tan) with hydrolyzed imipenem (gray) and the NDM-1 triple mutant (dark cyan). Hydrogen bonds are not shown for clarity. f Structural alignment from panel (e) with the wild-type NDM-1 structure removed but leaving the hydrolyzed imipenem from the wild-type structure in place. Thin black lines indicate hydrogen bonds. Thin red lines indicate close contacts with steric clash