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. 2018 Oct 30;9:4524. doi: 10.1038/s41467-018-06839-1

Table. 2.

Enzyme kinetic parameters for NDM-1 and mutants

Proteins Kinetic parameters Substratea
Ampicillin Cefotaxime Imipenem
NDM-1 KM (μM) 143 ± 3 6 ± 1 65 ±± 10
kcat (s−1) 550 ± 15 85 ± 16 238 ± 16
kcat/KM (μM−1 s−1) 3.84 ± 0.18 13.79 ± 1.49 3.71 ± 0.36
F64H KM (μM) 188 ± 31 6 ± 0.1 164 ± 19
kcat (s−1) 793 ± 24 83 ± 2 409 ± 15
kcat/KM (μM−1 s−1) 4.27 ± 0.57 14.80 ± 0.62 2.50 ± 0.20
D84E KM (μM) 82 ± 6 16 ± 0.2 70 ± 5
kcat (s−1) 597 ± 17 113 ± 3 153 ± 3
kcat/KM (μM−1 s−1) 7.29 ± 0.29 6.77 ± 0.23 2.20 ± 0.12
W87P KM (μM) 518 ± 36 6 ± 1 295 ± 54
kcat (s−1) 1823 ± 8 46 ± 3 351 ± 49
kcat/KM (μM−1 s−1) 3.53 ± 0.23 8.50 ± 1.84 1.20 ± 0.05
K121R KM (μM) 61 ± 3 4 ± 0.4 31 ± 9
kcat (s−1) 26 ± 0.35 13 ± 0.3 36 ± 2
kcat/KM (μM−1 s−1) 0.43 ± 0.03 3.69 ± 0.31 1.20 ± 0.28
K121T KM (μM) 231 ± 0.71 10 ± 2 117 ± 13
kcat (s−1) 246 ± 8 50 ± 0.3 50 ± 2
kcat/KM (μM−1 s−1) 1.06 ± 0.03 5.13 ± 0.79 0.43 ± 0.03
G195A KM (μM) 57 ± 14 4 ± 0.5 52 ± 15
kcat (s−1) 248 ± 24 32 ± 0.04 94 ± 11
kcat/KM (μM−1 s−1) 4.43 ± 0.67 7.74 ± 0.85 1.84 ± 0.31
D199E KM (μM) 11 ± 2 6 ± 0.4 6 ± 0.8
kcat (s−1) 23 ± 2 48 ± 0.7 17 ± 1
kcat/KM (μM−1 s−1) 2.10 ± 0.12 7.65 ± 0.40 2.81 ± 0.18
K224H KM (μM) 906 ± 26 26 ± 0.6 ND
kcat (s−1) 330 ± 6 111 ± 0.3 ND
kcat/KM (μM−1 s−1) 0.36 ± 0.004 4.24 ± 0.076 0.086 ± 0.002
K224R KM (μM) 179 ± 17 3 ± 0.2 69 ± 2
kcat (s−1) 294 ± 22 39 ± 2 62 ± 0.2
kcat/KM (μM−1 s−1) 1.65 ± 0.04 12.81 ± 0.28 0.90 ± 0.03
D225E KM (μM) 41 ± 9 4 ± 0.1 50 ± 8
kcat (s−1) 102 ± 0.8 38 ± 0.1 117 ± 8
kcat/KM (μM−1 s−1) 2.54 ± 0.56 9.78 ± 0.17 2.37 ± 0.24
G232A KM (μM) 178 ± 40 24 ± 2 ND
kcat (s−1) 417 ± 24 90 ± 1 ND
kcat/KM (μM−1 s−1) 2.39 ± 0.41 3.80 ± 0.25 0.63 ± 0.008
N233H KM (μM) 56 ± 3 3 ± 0.5 134 ± 43
kcat (s−1) 591 ± 6 55 ± 3 286 ± 30
kcat/KM (μM−1 s−1) 10.58 ± 0.61 20.42 ± 2.83 2.22 ± 0.49
N233Q KM (μM) 160 ± 13 1.1 ± 0.3 565 ± 84
kcat (s−1) 279 ± 23 25 ± 1 288 ± 31
kcat/KM (μM−1 s−1) 1.74 ± 0.01 22.95 ± 6.85 0.51 ± 0.02

ND not determined

aData are mean and standard deviations of at least two independent experiments