Figure 1.

Effects of recombinant NiV N expression on NiV replication. Cells were transfected with increasing amounts of plasmid DNA encoding the NiV N gene. (a) 48 hours post transfection, cells were harvested and analyzed by western blot using a monoclonal antibody against NiV N. Western blots were quantified by densitometry and normalized to actin. Following transfection, a parallel set of cells were infected with NiV at an MOI of 1 for 24 hours. (b) RNA was extracted from NiV infected cells and analyzed by northern blot using a probe designed against the M gene. Northern blots were quantified by densitometry and normalized to GAPDH. (c) Total protein was harvested from NiV infected cells and analyzed by western blot for the expression of NiV P proteins. Western blots were quantified by densitometry and normalized to actin. (d) Supernatants were harvested, viral loads were determined by endpoint titration and TCID₅₀/ml was calculated. All experiments were carried out in triplicate. Standard deviations of the mean were calculated. Blots have been cropped to ease visualization.