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. 2018 Oct 30;8:15994. doi: 10.1038/s41598-018-34484-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of increasing expression of recombinant proteins on viral translation. Cells were transfected with increasing amounts of plasmid DNA encoding the (a) NiV P gene, (c) NiV F gene, or (e) GFP gene. 48 hours post transfection, cells were harvested and analyzed by western blot. Protein expression was quantified by densitometry and normalized to actin. Following transfection, a parallel set of cells were infected with NiV at an MOI of 1 for 24 hours. Total protein was harvested from NiV infected cells and analyzed by western blot for the expression of (b) NiV N proteins or (d) and (f) NiV P proteins. Western blots were quantified by densitometry and normalized to actin. All experiments were performed in triplicate and standard deviations of the mean were calculated. Blots have been cropped to ease visualization.