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. 2018 Oct 30;8:15994. doi: 10.1038/s41598-018-34484-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of recombinant NiV N expression on input polymerase activity. Cells were transfected with increasing amounts of plasmid DNA encoding the NiV N gene. Total RNA was extracted from infected cells at (a) 24 hpi, (b) 0, 6 and 9 hpi, and 1 µg of RNA was analyzed by strand-specific RT and real-time PCR spanning the intergenic region between the NiV G and L genes. Each reaction was standardized to the input genome copy number after a 1 hour adsorption of virus and the ratio between genome copy numbers and input genome copy numbers was plotted. All experiments were carried out in triplicate. Standard deviations of the mean were calculated.