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. 2018 Oct 24;9:1531. doi: 10.3389/fpls.2018.01531

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Copyright © 2018 Buendia, Girardin, Wang, Cottret and Lefebvre.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PhyML phylogenetic tree of the LYMs. Different phylogenetic groups are shown in different colors. ECRs of 3 LYR proteins were used as outgroup sequences. The sequences corresponding to the LYM proteins were aligned with Mafft (v7.271; Katoh et al., 2002) with the following parameters: maxiterate = 1000, retree = 1, genafpair = true. The best evolutionary model fitting the alignment was identified using ProtTest (v2.4). This best model was: WAG + I + G, alpha = 2.69, p-inv = 0.05. The phylogenetic tree was computed using a maximum-likelihood method with phyml (v20130805; Guindon et al., 2010). The branch confidence was evaluated using the Approximate Likelihood-Ratio Test (Anisimova and Gascuel, 2006). Finally, the tree was drawn with Itol v3 (Letunic and Bork, 2016).