Fig. 5.

Severe hypoglycemia and depleted glycogen stores without alterations in lactate, free fatty acids and hepatic gluconeogenic enzyme expression in infected Adx mice. Sham and Adx C57BL/6 or BALB/c mice were infected with PcAS (a–g) or PbNK65-E (h–k), respectively. Infected mice were euthanized and dissected at 8 to 10 days p.i. (PcAS) or 6 to 7 days p.i (PbNK65-E). a,h Glucose levels were determined in plasma (detection limit was 20 mg/dL, indicated by dotted line). Samples with a measurement below the detection limit, were given an arbitrary value of 10 mg/dL. b, c Blood lactate and plasma free fatty acid levels were measured. The dotted lines represent the limits of detection. d Hepatic glycogen content (in mg/g of tissue). (e–g, i–k) Livers were homogenized and specific mRNA levels were measured by qRT-PCR. Each symbol represents data from an individual mouse, (d) n = 14 for PcAS Adx. Horizontal lines in between data points represent group medians and analysis was by Mann-Whitney U-test. Horizontal lines with asterisks on top indicate the levels of statistical significance between the indicated groups. Asterisks above individual data sets indicate the levels of statistical significance compared to the uninfected control group. Data from at least two separate experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, ****p < 0.0001