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. 2018 Oct 30;8:16006. doi: 10.1038/s41598-018-34475-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of long-term Dex treatment on growth and colony forming ability of model lung adenocarcinoma cell lines. H292, A549, H1299, H1299GR Clone 2 and H1299GR Clone 4 cells were treated with either vehicle or Dex (100 nM) and cell growth was monitored by counting the viable cells using the Trypan blue dye exclusion assay (Panel A). The same cell lines were treated with Dex (100 nM) for the indicated days and then plated to measure colony formation without further treatment (Panel B). H1299GR Clone 4 cells or parental H1299 cells were treated with vehicle or the indicted concentrations of Dex for 7 days and plated to measure colony formation without further treatment (Panel C). Panel A: *P, 0.0001. Panel B (A549 cells): *P, 0.0001. Panel B (H292 cells): *P, 0.001. Panel B (H1299GRα clone2): *P, 0.001; ^§P, 0.0005; ^‡P, 0.015. Panel B (H1299GRα clone4): *P, 0.001; ^§P, 0.0024; ^‡P, 0.0001. Panel C: *P, 0.00004; **P, 0.0008; ^§P, 0.0009; ^‡P, 0.0005.