Table 2.

Histopathologic findings in 16 NHPs positive for Zika virus.

Sample ID	Cerebrum	Heart	Lung	Liver	Spleen	Kidney
PR 17/03	Autolysis	Autolysis	Autolysis	Autolysis	Autolysis	Autolysis
PR 17/04	Autolysis	Autolysis	Mild multifocal suppurative bronchopneumonia; Intravascular microfilariae	Moderate multifocal chronic proliferative cholangitis with intralesional trematode ova and adults; Mild to moderate diffuse vacuolar (hydropic) hepatopathy; Intravascular microfilariae	Autolysis	Minimal focal lymphoplasmacytic interstitial nephritis
PR 17/05	Congestion and multifocal acute hemorrhage.	Congestion and multifocal acute hemorrhage.	Multifocal acute alveolar hemorrhage	Multifocal random mononuclear inflammatory infiltrates and minimal pericholangitis; Mild diffuse vacuolar (hydropic) hepatopathy	Mild to moderate, diffuse sinus histiocytosis	Minimal multifocal acute tubular degeneration with mild proteinosis; Minimal focal chronic lymphocytic interstitial nephritis
PR 17/07	NSLO	NSLO	Moderate to severe acute alveolar hemorrhage	Marked, diffuse vacuolar (hydropic) hepatopathy	Extramedullary hematopoiesis; Lymphoid reactive hyperplasia	Without NSLO
PR 17/08	NE	Minimal multifocal chronic lymphocytic myocarditis	Marked multifocal acute alveolar and bronchial hemorrhage with edema and histiocytosis; Mild multifocal chronic lymphocytic and eosinophilic perivasculitis and mild lymphoid hyperplasia; Focal parabronchial arterial bone marrow embolus.	Congestion; Minimal, focal, chronic eosinophilic pericholangitis and minimal reactive changes.	Diffuse lymphoid reactive hyperplasia and sinus histiocytosis	Mild, multifocal tubular proteinosis; Congestion.
PR 17/11	Multifocal acute cortical neuron necrosis	Congestion, edema and focal hemorrhage	Congestion, edema and focal hemorrhage	Moderate diffuse vacuolar (hydropic) hepatopathy	Diffuse lymphoid reactive hyperplasia	Moderate multifocal chronic membranous glomerulonephritis with lymphoid follicle formation
PR 17/12	NSLO	NSLO	Moderate multifocal acute alveolar hemorrhage and edema	Moderate diffuse vacuolar (hydropic) hepatopathy; Moderate multifocal neutrophilic portal hepatitis	Mild diffuse lymphoid reactive hyperplasia	Congestion
PR 17/13	Mild focal hemorrhage	Mild multifocal acute myocyte degeneration	Mild multifocal acute interstitial pneumonia	Marked multifocal chronic proliferative cholangiohepatitis with marked cholestasis and necrosis; Extramedullary hematopoiesis	Multifocal acute necrotizing splenitis; Extramedullary hematopoiesis.	NSLO
PR 17/14	Congestion	NSLO	Moderate multifocal subacute interstitial pneumonia with bronchiolitis	NSLO	Moderate lymphoid depletion	NSLO
PR 17/15	NE	NSLO	Autolysis	NSLO	NSLO	Mild multifocal chronic interstitial nephritis
PR 17/16	Congestion	Minimal focal chronic lymphocytic infiltrate; Congestion	Diffuse atelectasia; Mild multifocal subacute suppurative bronchopneumonia	Mild multifocal chronic lymphocytic pericholangitis; Mild diffuse vacuolar (hydropic) hepatopathy	NE	Mild multifocal chronic lymphocytic interstitial nephritis; Mild multifocal acute tubular degeneration
PR 17/18	Minimal focal lymphocytic vascular cuffing; Congestion and multifocal alveolar hemorrhage	Mild multifocal myocardial fibrosis; Mild multifocal acute myocyte degeneration with contraction band necrosis	Multifocal acute alveolar hemorrhage	Mild multifocal chronic eosinophilic and lymphoplasmacytic cholangitis with intraductal adult trematodes, bile duct hyperplasia and mild fibrosis	Mild diffuse lymphoid reactive hyperplasia and sinus histiocytosis	Mild multifocal chronic lymphoplasmacytic interstitial nephritis; Mild multifocal segmental membranous glomerulonephritis with proteinuria; Mild multifocal acute tubular necrosis
PR 17/19	Multifocal acute hemorrhage and congestion.	NSLO	Congestion, edema and multifocal acute alveolar hemorrhage; Focal interstitial nodular lymphohistiocytic and eosinophilic infiltrate	Mild multifocal chronic lymphoplasmacytic pericholangitis	Diffuse lymphoid reactive hyperplasia and histiocytosis	Moderate diffuse global chronic membranoproliferative glomerulitis with moderate lymphoplasmacytic interstitial nephritis; Focal granuloma.
PR 17/20	NE	NSLO	Locally extensive alveolar hemorrhage; Moderate focal acute suppurative bronchopneumonia	Mild diffuse vacuolar (hydropic) hepatopathy	NSLO	NSLO
PR 17/21	Mild focal chronic lymphocytic meningitis	Congestion	Diffuse congestion, edema and atelectasia; mild alveolar hemosiderosis	Mild multifocal chronic lymphoplasmacytic and granulomatous pericholangitis with mild hemosiderosis; Mild multifocal acute single cell hepatocellular necrosis	Mild diffuse lymphoid reactive hyperplasia and mild sinus histiocytosis	Mild multifocal acute tubular degeneration with proteinosis e atrofia glomerulocística focal
PR 17/22	NSLO	NSLO	Diffuse congestion, edema and multifocal acute hemorrhage; Multifocal atelectasia; Mild lymphoplasmacytic peribronchial and perivascular infiltrates with rare alveolar multinucleate giant cells	Mild multifocal chronic lymphoplasmacytic and eosinophilic cholangiohepatitis with moderate bile duct hyperplasia; Mild diffuse vacuolar (hydropic) hepatopathy	Moderate diffuse lymphoid reactive hyperplasia and sinus histiocytosis	Minimal multifocal chronic lymphoplasmacytic interstitial nephritis and rare glomerulocystic atrophy

NSLO: no significant lesions observed; NE: not evaluated.