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. 2018 Oct 30;8:16054. doi: 10.1038/s41598-018-34326-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Genomic DNA sequence of the first exon and the upstream intron region within the IGFBP-2b1 and IGFBP-2b genes. The exon is in standard text while the intron is bold. The target crRNA annealing regions are highlighted in gray and the adjacent PAM site is underlined. Primer annealing sites for detection of indels by PCR are shown with the solid underline. Primer annealing sites for sequencing are shown with the dashed underline.