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. 2018 Oct 30;8:16054. doi: 10.1038/s41598-018-34326-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histograms indicating the frequency distribution of the IGFBP-2b phenotype (panel a: mutants, panel b: controls) and mutant genotype (mutants: panel c). The solid horizontal lines represent the separation of the mutants into high-rate (<50% IGFBP-2b) and low-rate (>50% IGFBP-2b) mutant groups. The dashed horizontal line in panel a represents the range of IGFBP-2b values in control fish while the vertical arrow represents the control mean. The values on the x-axis represent the lower and upper boundary for each bin. ADU: arbitrary density units.