Table 1.

Summary of total and mutant reads for IGFBP-2b1 and IGFBP-2b2 for a single control (#C-75) and each mutant fish.

Fish #	IGFBP-2b1					IGFBP-2b2					Average Mutant %	Average Frame-shift %	IGFBP-2b Abundance (ADU)
	Target Reads	Mutant Reads	Mutant %	Frameshift Mutant Reads	Frame-shift %	Target Reads	Mutant Reads	Mutant %	Frameshift Mutant Reads	Frame-shift %
C-75	102205	439	0.43	208	0.20	73599	315	0.43	315	0.43	0.43	0.32	4770.6
M-38	130384	12820	9.83	6658	5.11	75747	1630	2.15	1292	1.71	5.99	3.41	2040.0
M-39	106473	93191	87.53	67537	63.43	93341	54711	58.61	53338	57.14	73.07	60.29	606.9
M-42	117540	82728	70.38	54061	45.99	99031	79321	80.10	69191	69.87	75.24	57.93	2138.1
M-45	100114	86525	86.43	21788	21.76	112178	76858	68.51	68638	61.19	77.47	41.48	796.9
M-46	110024	57975	52.69	10726	9.75	94621	20416	21.58	19719	20.84	37.14	15.30	2543.8
M-47	105896	105599	99.72	16436	15.52	107615	57443	53.38	48417	44.99	76.55	30.26	132.1
M-48	116452	71837	61.69	31584	27.12	114099	36394	31.90	35148	30.80	46.80	28.96	2379.6
M-49	98969	98887	99.92	70078	70.81	81599	67339	82.52	47132	57.76	91.22	64.29	NA
M-56	94822	81564	86.02	9052	9.55	92340	60122	65.11	53173	57.58	75.57	33.57	720.2
M-59	119493	57773	48.35	42019	35.16	94932	32508	34.24	32508	34.24	41.30	34.70	3192.6
M-62	106914	93317	87.28	9637	9.01	118782	70794	59.60	61009	51.36	73.44	30.19	220.7
M-63	106686	81489	76.38	67488	63.26	93837	43035	45.86	42660	45.46	61.12	54.36	407.5
M-64	89830	43978	48.96	12900	14.36	108598	30162	27.77	29792	27.43	38.37	20.90	2163.1
M-73	71027	5598	7.88	1166	1.64	94429	2812	2.98	2708	2.87	5.43	2.26	3289.9
M-82	88604	56730	64.03	51520	58.15	103346	55836	54.03	53925	52.18	59.03	55.17	860.9
M-89	95525	26763	28.02	9263	9.70	122216	31536	25.80	30544	24.99	26.91	17.35	3057.2
M-90	109703	104315	95.09	29700	27.07	109751	89246	81.32	86991	79.26	88.21	53.17	516.4
M-93	86706	58523	67.50	26839	30.95	94676	52436	55.38	22596	23.87	61.44	27.41	1206.1
M-95	100239	27951	27.88	21601	21.55	90964	42868	47.13	11029	12.12	37.51	16.84	2062.3
M-96	111179	95667	86.05	59567	53.58	101967	68983	67.65	66613	65.33	76.85	59.46	644.5
M-97	80600	78020	96.80	57203	70.97	73712	54927	74.52	49361	66.96	85.66	68.97	545.5
M-106	71828	70874	98.67	20493	28.53	107918	72139	66.85	60598	56.15	82.76	42.34	243.9
M-107	87747	68541	78.11	45343	51.67	85947	37473	43.60	35938	41.81	60.86	46.74	916.1
M-108	96546	88737	91.91	25460	26.37	112393	56845	50.58	53891	47.95	71.25	37.16	772.1

The number and percent reads that result in frameshift mutations are also indicated. Averaged across all samples, 99.7% of the filtered reads from the IGFBP-2b1 PCR mapped to the IGFBP-2b1 reference sequence (range: 96.5%–99.9%). Similarly, 99.86% of the filtered reads from the IGFBP-2b2 PCR mapped to the IGFBP-2b2 target reference (range: 98.5%–99.9%). ADU: arbitrary density units.