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. 2018 Oct 30;8:16054. doi: 10.1038/s41598-018-34326-6

Table 2.

Gene variants detected in Fish #M-90 at greater than 1% frequency.

Fish #M-90: Gene Variants Type Percent of Total Reads Indel Length
IGFBP-2b1
TCTGTGGCGTCTACACACCGAGGTGTT–CAGGGCTGC Deletion 60.26 −3
TCTGTGGCGTCTACACACCGAGGTGTGtagacgacagggctgcgttgtcacacCGACAGGGCTGC Insertion 16.57 26
TCTGTGGCGTCTACACACCGAGGTGTTCCACAGGGCTGC Ref Seq 4.65 0
TCTGTGGCGTCTACACACCGAGGT-----------CTGC Deletion 4.14 −11
TCTGTGGCGTCTACACAC------------CAGGGCTGC Deletion 1.63 −12
TCTGTG-------------------------------GC Deletion 1.44 −31
TCTGTGGCGTCTACACACCGAGGTGT------GGGCTGC Deletion 1.18 −6
IGFBP-2b2
ACATTTCTTGCTCTGCCGGTTCTATT----GGGGGCATGG Deletion 29.05 −4
ACATTTCTTGCTCTGCCGGTTCTATTG–CGGGGGCATGG Deletion 20.90 −2
ACATTTCTTGCTCTGCCGGTTCTATTGC-AGGGGGCATGG Deletion 16.25 −1
ACATTTCTTGCTCTGCCGGTTCTATTGCTCGGGGGCATGG Ref Seq 13.31 0
ACATTTCTTGCTCTGCCGGTTCTATT--------GCATGG Deletion 7.89 −8
ACATTTCTTGCTCTGCCGGTTCTATTGCATGGTGGCATGG 3 Base Changes 3.62 0
ACATTTCTTGCTCTGCCGGTTCTATTGCT–GGGCATGG Deletion 1.94 −3
ACATTTCTTGCTCTGCCGGTTCTATTGCATGGGGGCATGG 2 Base Changes 1.09 0

Bold indicate insertions and base pair substitutions compared to the reference sequence (Ref Seq). The crRNA target annealing site is underlined.