Table 2.
Fish #M-90: Gene Variants | Type | Percent of Total Reads | Indel Length |
---|---|---|---|
IGFBP-2b1 | |||
TCTGTGGCGTCTACACACCGAGGTGTT–CAGGGCTGC | Deletion | 60.26 | −3 |
TCTGTGGCGTCTACACACCGAGGTGTGtagacgacagggctgcgttgtcacacCGACAGGGCTGC | Insertion | 16.57 | 26 |
TCTGTGGCGTCTACACACCGAGGTGTTCCACAGGGCTGC | Ref Seq | 4.65 | 0 |
TCTGTGGCGTCTACACACCGAGGT-----------CTGC | Deletion | 4.14 | −11 |
TCTGTGGCGTCTACACAC------------CAGGGCTGC | Deletion | 1.63 | −12 |
TCTGTG-------------------------------GC | Deletion | 1.44 | −31 |
TCTGTGGCGTCTACACACCGAGGTGT------GGGCTGC | Deletion | 1.18 | −6 |
IGFBP-2b2 | |||
ACATTTCTTGCTCTGCCGGTTCTATT----GGGGGCATGG | Deletion | 29.05 | −4 |
ACATTTCTTGCTCTGCCGGTTCTATTG–CGGGGGCATGG | Deletion | 20.90 | −2 |
ACATTTCTTGCTCTGCCGGTTCTATTGC-AGGGGGCATGG | Deletion | 16.25 | −1 |
ACATTTCTTGCTCTGCCGGTTCTATTGCTCGGGGGCATGG | Ref Seq | 13.31 | 0 |
ACATTTCTTGCTCTGCCGGTTCTATT--------GCATGG | Deletion | 7.89 | −8 |
ACATTTCTTGCTCTGCCGGTTCTATTGCATGGTGGCATGG | 3 Base Changes | 3.62 | 0 |
ACATTTCTTGCTCTGCCGGTTCTATTGCT–GGGCATGG | Deletion | 1.94 | −3 |
ACATTTCTTGCTCTGCCGGTTCTATTGCATGGGGGCATGG | 2 Base Changes | 1.09 | 0 |
Bold indicate insertions and base pair substitutions compared to the reference sequence (Ref Seq). The crRNA target annealing site is underlined.