Table 1.
Pollen viability of transformed and control plants.
Plant | H | E | PN | NVP | VP | PVP (95% CI) |
---|---|---|---|---|---|---|
#RNAi1 | + | + | 4765 | 636 | 4129 | 0.8675 (0.8564 < p < 0.876) |
#RNAi2 | + | + | 1833 | 293 | 1540 | 0.8402 (0.8224 < p < 0.8565) |
#TC1 | - | + | 2428 | 298 | 2130 | 0.8773 (0.8634 < p < 0.89) |
#TC2 | - | + | 1238 | 118 | 1120 | 0.9106 (0.8929 < p < 0.9257) |
#Q4117 | - | - | 997 | 100 | 897 | 0.8997 (0.879 < p < 0.9173) |
H, QUI-GON hairpin; E, eGFP; PN, total number of counted pollen grains; NVP, number of non-viable pollen grains; VP, number of viable pollen grains; PVP, proportion of viable pollen. 95% CI (confidence intervals) were calculated by using the Newcombe method (Newcombe, 1998), with a correction for continuity (http://vassarstats.net/prop1.html), as described in the section “Materials and Methods.”