Table 2.
Cytoembryological analysis of transformed and control plants.
| Plant | H | E | N | Ab | V | AES | AES/N | KW (ranks/group) | MES | MES/N (95% CI) |
|---|---|---|---|---|---|---|---|---|---|---|
| #RNAi1 | + | + | 40 | 17 | 23 | 41 | 1.02 | 50.54/A | 9 | 0.225 (0.114 < p < 0.388) |
| #RNAi2 | + | + | 43 | 9 | 34 | 54 | 1.25 | 57.38/A | 8 | 0.186 (0.089 < p < 0.339) |
| #TC1 | - | + | 24 | 1 | 23 | 82 | 3.41 | 111.69/B | 4 | 0.166 (0.0548 < p < 0.381) |
| #TC2 | - | + | 21 | 3 | 18 | 100 | 4.76 | 131.64/B | 6 | 0.285 (0.121 < p < 0.523) |
| #Q4117 | - | - | 42 | 4 | 38 | 142 | 3.38 | 109.55/B | 9 | 0.214 (0.108 < p < 0.372) |
H, QUI-GON hairpin; E, eGFP; N, number of ovules analyzed; Ab, aborted ovules (without embryo sacs); V, potentially viable ovules (with sacs); AES, total number of aposporous embryo sacs; AES/N, average number of aposporous embryo sac per ovule; KW, Test of Kruskal–Wallis comparing the AES per ovule variable; MES, total number of meiotic embryo sacs; MES/N, average number of meiotic embryo sacs per ovule; 95% CI, ninety-five % confidence intervals around MES/N proportions were calculated by using the Newcombe method (Newcombe, 1998) with a correction for continuity (http://vassarstats.net/prop1.html), as described in the section “Materials and Methods.” Aposporous embryo sacs (AES) can be readily distinguished from meiotic ones (MES) since they lack antipodal cells.