Fig. 2.

Brain uptake of [³H]taurocholate following 6-hour treatment with BMP-9. (A) Effect of BMP-9 treatment on OATP-mediated uptake of [³H]taurocholate was measured via a multiple-times uptake in situ brain perfusion study. Animals were treated with BMP-9 (1 μg/kg, i.p.; 6 hours) in the presence and absence of LDN193189 (10 mg/kg, i.p.; 1-hour pretreatment) and perfused with [³H]taurocholic acid (1.0 μCi/ml) for 2.5, 5, 10, and 20 minutes. (B) AUC analysis was conducted on multiple-times uptake data using the trapezoidal method to obtain an indicator of CNS total drug exposure. Results are expressed as mean ± S.D. of six animals per time point. Asterisks represent data points that were significantly different from control (saline-treated) animals (*P < 0.05; **P < 0.01).