Fig. 3.

Brain accumulation of [³H]taurocholate is determined by OATP-mediated transport at the BBB. Effect of established OATP transport inhibitors (i.e., E3S and FEX) on the uptake of [³H]taurocholate into rat brain post-treatment was assessed by in situ brain perfusion studies. [³H]taurocholate accumulation was measured in animals injected with BMP-9 (1 μg/kg, i.p.) in the presence and absence of LDN193189 (10 mg/kg, i.p.; 1 hour pretreatment) where animals were perfused for 10 minutes with E3S (100 μM) or FEX (100 μM) or BSP (1 mM) prior to perfusion with [³H]taurocholic acid. Results are expressed as mean ± S.D. of six animals per treatment group. Data points that are significantly different are indicated as follows: **P < 0.01.