Fig. 8.

TGF-β/ALK1 signaling is involved in regulation of Slco1a4 mRNA transcripts in rat brain microvessels following BMP-9 treatment. ChIP was performed on brain microvessels isolated from animals treated with 0.9% saline, BMP-9 alone (1 μg/kg, i.p.; 6 hours) or BMP-9 in the presence of LDN193189 (10 mg/kg, i.p.; 1-hour pretreatment). Sheared chromatin was immunoprecipitated using Smad4 antibody, and quantification of Smad4 complex binding to specific Smad1/5/8 binding sites within the Slco1a4 gene promoter was performed by real-time PCR. Results are expressed as mean ± S.D. of three experiments, with each group consisting of microvessels from three individual animals. Asterisks represent data points that were significantly different from control (saline-treated) animals (**P < 0.01).