TABLE 1.

Kinetic analysis of multiple-times uptake data of Oatp1a4-mediated [³H]taurocholate transport in rat brain

Animals were treated with BMP-9 (1 μg/kg, i.p.) in the presence and absence of LDN193189 (10 mg/kg, i.p.; 1-hour pretreatment) and perfused with [³H]taurocholic acid (1.0 μCi/ml) for 2.5, 5, 10, and 20 minutes. Results are expressed as mean ± S.D. of six animals per treatment group. Asterisks represent data points that were significantly different from control (saline-treated) animals (**P < 0.01).

Parameter	Control	BMP-9	LDN193189 + BMP-9
VBR (pmol/g)	89.89 ± 3.69	172.20 ± 7.19**	82.85 ± 3.16
KIN (pmol/g⋅min)	9.37 ± 1.15	14.34 ± 1.66**	9.18 ± 0.77
Kout (min−1)	0.104 ± 0.031	0.083 ± 0.023	0.111 ± 0.024