Figure 5.
qPCR analysis of peripheral blood gene expression at baseline according to IFNL4 genotype. A set of genes was quantified by PCR from RNA derived from peripheral blood using the relative quantification method with genes normalized against a housekeeping gene (GAPDH). Results from eight ISG gene expressions are shown in a total of 45 patients from the same cohort, of which 27 were CC and 18 patients were non‐CC genotype. Results from qPCR analysis were fed into GraphPad Prism (7.0; GraphPad Software Inc., La Jolla, CA) to generate the graphs. P values were generated using the statistical software within Prism using log values of fold changes and SEM is shown. Abbreviation: GAPDH, glyceraldehyde 3‐phosphate dehydrogenase.