Subconfluent CFPAC and PCSC cells were sent for analysis of C1P. Lipidomic analysis of C1P normalized to the specific isoforms standard curve, determined that C1P species d18:1/18:0-C1P (CFPAC: 41ρm ± 10SEM; PCSC:4.3ρm ± 0.2SEM, * p<0.007), d18:1/18:1-C1P (CFPAC: 48.3ρm ± 9SEM; PCSC:5.8ρm ± 0.7SEM, ** p=0.002), and d18:1/20:0-C1P (CFPAC: 18.8ρm ± 3.6SEM; PCSC:0.5ρm ± 0.1SEM, * p<0.007) were significantly increased in CFPAC versus PCSC (unpaired Student’s t-test, n= 3/experiments performed on different cell passages harvested at different times and normalized to total cell number) (nomenclature: Sphingosine backbone of C1P is - 18:1).