Figure 4:

C1P increases FN-mediated PCSC adhesion. Wells coated with fibronectin (FN, 1–10μM), collagen (CL,1–10μM), laminin (LA, 1–10μM), or vehicle (CAPS) as indicated while some wells were dual-coated with C1P (10μM) or ceramide (10μM). PCSC or CFPAC cells were seeded on pre-coated plates and adherent cells identified by crystal violet staining. PCSC cells demonstrated dose-dependent adhesion to FN (A), CL (B) and LA (C). CFPAC cells were non-adherent to FN (data not shown) but demonstrated a dose-dependent adhesion to CL (D). C1P significantly enhanced PCSC adhesion to 10 μM FN (A, 0.6145 ± 0.0272 SEM) (p=0.04, n=4/condition/experiment performed a minimum of 4 occasions) as compared to 10 μM FN alone (0.4563 ± 0.0570 SEM) or ceramide ± 10 μM FN (0.3440 ± 0.0032 SEM).