Figure 2.

Role of integrin αvβ5 in the development of immune cells. (A) Generation of Itgb5^−/−→WT BM chimeras. (B) Frequency among CD45.2⁺ leukocytes (top panels) and absolute numbers (lower panels) of colonic macrophage subsets, together with monocytes in peripheral blood of Itgb5^−/−→WT and WT→WT chimeric mice, determined by flow cytometry. Horizontal bars represent the medians and each symbol represents an individual mouse. Data pooled from three independent experiments with 4–5 mice/experiment. *p < 0.05, ***p < 0.001, two-tailed Mann–Whitney test. (C) Generation of CD45.1⁺Ccr2^−/−:CD45.2⁺WT and CD45.1⁺Ccr2^−/−:CD45.2⁺Itgb5^−/− mixed BM chimeras. (D) Representative FACS staining of colonic macrophage subsets showing chimerism 8 weeks after reconstitution. (E) Frequencies of donor-derived CD45⁺ leukocytes amongst colonic monocyte and macrophage subsets in CD45.1⁺Ccr2^−/−:CD45.2⁺WT and CD45.1⁺Ccr2^−/−:CD45.2⁺Itgb5^−/− mixed BM chimeras. Horizontal bars represent the medians and each symbol represents an individual mouse. Data pooled from two independent experiments with 3–4 mice/experiment. *p < 0.05, **p < 0.01, two-tailed Mann–Whitney test.