Fig. 1.

EGFP⁺ myeloid cells in the brain after hypoxia-ischemia. a–c Gating strategy applied to all samples displaying cells isolated from the ipsilateral hemisphere at 24 h after hypoxia-ischemia (HI). Single-cell suspensions derived from ipsilateral and contralateral hemispheres of Lys-EGFP-ki mice were gated based on size (forward scatter) and granularity (side scatter) (a) followed by CD11b immunoreactivity (b) and EGFP expression (c). d EGFP⁺ cells display CD45^hi expression; n = 12. e, f Backgating shows CD11b⁺EGFP⁺ in the contralateral (e) and ipsilateral (f) hemispheres 24 h after HI. g Compiled data displaying presence of CD11b⁺EGFP⁺ infiltrating cells at 6 h (n = 8), 1 day (n = 7), 3 days (n = 23), 7 days (n = 19), 14 days (n = 14) and 28 days (n = 9) after HI. Values are presented as the mean ± SD. One-way ANOVA followed by Holm-Sidak’s post hoc test comparing differences between hemispheres at each time point. *p ≤ 0.05, ***p ≤ 0.001