Fig. 6.

Depletion of myeloid cells in the injured CNS. a–c Using the same gating strategy as described in Fig. 1a–c and Fig. 4a, EGFP⁺ cells in the brain after saline injection (b) or RB6-8C5 antibody administration (c) were identified as resident monocytes (CD11b⁺EGFP⁺Ly6C^lo/−), inflammatory monocytes (CD11b⁺EGFP⁺Ly6C^hi) and granulocytes (CD11b⁺EGFP⁺Ly6C^int). d–g Compiled data displays the effect of RB6-8C5 antibody administration on the overall CD11b⁺EGFP⁺ cell population (d) resident monocytes (e), inflammatory monocytes (f) and granulocytes (g), at 1 day (Sal: n = 6; RB6-8C5: n = 3) and 7 days (Sal: n = 12; RB6-8C5: n = 13) after hypoxia-ischemia in the ipsilateral and contralateral hemispheres. Values are presented as mean ± SD. One-way ANOVA followed by Holm-Sidak’s post hoc test was used for comparing the differences between the contralateral and ipsilateral hemispheres at different time points. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001