Table 1.
Antibodies for immunohistochemistry and flow cytometry
| Application | Antigen | Host | Clone/target | Reactivity* | Conjugate | Company | Product number | Dilution** |
|---|---|---|---|---|---|---|---|---|
| Immunohistochemistry | GFP | Rabbit | Poly | Mouse | A488 | Invitrogen | A21311 | 1:200 |
| CD31 | Goat | Poly | Mouse | – | R&D systems | AF3628 | 1:200 | |
| Iba1 | Goat | Poly | Mouse | – | Abcam | Ab5076 | 1:500 | |
| Ly6G | Rat | Mono (1A8) | Mouse | – | BioLegend | 127602 | 1:250 | |
| IgG | Donkey | Poly | Goat | CF555 | VWR | 89138-464 | 1:1000 | |
| IgG | Goat | Poly | Rat | CF555 | Sigma | SAB46000070 | 1:1000 | |
| Flow cytometry | CD11b | Rat | M1/70 | Mouse | PE-Cy7 | BioLegend | 101216 | 0.25 μg |
| CD45 | Rat | 30-F11 | Mouse | APC-CY7 | BD | 557659 | 0.25 μg | |
| GR-1 | Rat | RB6-8C5 | Mouse | PerCp-Cy5.5 | eBiosciences | 45-5931 | 0.25 μg | |
| Ly6C | Rat | HK1.4 | Mouse | APC | eBiosciences | 17-5932 | 0.25 μg | |
| CD16/CD32 | Rat | 2.4G2 | Mouse | – | BD | 553142 | 0.1 μg |
*Where antibody is reactive to multiple species, only relevant species are listed
**For flow cytometry, given values represent microgram antibody per 106 cells