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. 2018 Oct 30;18:1055. doi: 10.1186/s12885-018-4980-7

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Western Blot of DNMT3A, CDC42 (a) and APC (b) proteins in control and transfected cells of AGP01, ACP02 and ACP03 cell lines. (a) DNMT3A and CDC42 were down-regulated after the transfection of mimics of hsa-miR-29c-5p in AGP01 and ACP03 2D cell lines. There was no difference in the expression of both proteins in transfected cells of ACP02 2D cell line. b APC was up-regulated in all three 2D cell lines after the transfection of hsa-miR-135b-5p antimiRs. β-Actin was used as a loading control in all blot experiments. NC stands for Negative Control; M for transfected with mimics; and Am for transfected with antimiRs