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. Author manuscript; available in PMC: 2018 Oct 31.
Published in final edited form as: J Invest Dermatol. 2016 Oct 6;137(2):457–465. doi: 10.1016/j.jid.2016.09.022

Figure 5. Quantification of acceptor photobleaching FRET.

Figure 5.

Wildtype or mutant GPR143-EYFP and TYR-ECFP were co-transfected in COS7s. (a) Ratio of emission intensity after:before bleaching. Controls = single transfected COS7s, TYR-ECFP + A2AAR, and ECFP-EYFP fusion. For wtGPR143, photobleaching and relative quantification was performed in intracellular regions. For mtGPR143, portions of plasma membrane were analyzed. (b) FRET efficiency was quantified for co-transfected COS7s. Controls = Single transfected cells, TYR-ECFP + A2AAR, and ECFP-EYFP fusion protein. Data represent means ±SEM of four (co-transfected cells), three (TYR-ECFP + A2AAR) or two control cells. Unpaired Student t-test: * p<0.05, ** p<0.01, ns not significantly different from control. Values refer to limited regions (See Figures S6, S7, S8). A2AAR, adenosine A2A receptor; mt, mutant; TYR, tyrosinase; wt, wildtype.