Figure 6.
Mitochondrial uncoupling in Leishmania tarentolae promastigotes (LtP) in the presence of major components of essential oil from Chenopodium ambrosioides L. Uncoupling was assessed by stimulation of oxygen consumption in the presence of the ATP synthase inhibitor oligomycin (Oligo). LtP cells (1 × 108/ml) in brain heart infusion medium were supplemented with Oligo (5 μM) and decreasing concentrations of ascaridole (Asc), carvacrol (Car), caryophyllene oxide (Caryo), and the uncoupler CCCP. Oxygen consumption was assessed in 96‐well OxoPlates for 40 min at 26 °C. Oxygen consumption rates were normalized to the respiration of non‐inhibited Leishmania (LtP = 100%). Data represent mean ± standard deviation of 3–4 experiments. *Significant differences versus LtP + Oligo on the level p < .05