Table 1.
Influence of major EO components on the LtP‐Mit in comparison with BH‐SMP on mitochondrial activities of complexes I–III
| Compound | IC50 (μM; % of inhibition at highest concentration tested) | |||||
|---|---|---|---|---|---|---|
| Complex I | Complex II | Complex III | ||||
| LtP‐Mit | BH‐SMP | LtP‐Mit | BH‐SMP | LtP‐Mit | BH‐SMP | |
| Asc | >100 (1.1) | >100 (5.1) | >100 (17.6) | >100 (21.2) | 131.3 ± 3.5 | >100 (17.7) |
| Car | >100 (16.4) | >100 (13.3) | >100 (8.8) | >100 (21.5) | 150.1 ± 2.3 | >100 (22.8) |
| Caryo | >100 (7.2) | >100 (39.7) | >100 (12.3) | >100 (16.3) | 54.8 ± 4.0 | >100 (10.0) |
Note. IC50 > value: In these cases, the IC50 was not determined because only an inhibition <50% was observed at highest concentration. In parentheses, % of inhibition at highest concentration tested. Results were expressed as mean ± SD or as percentage of three independent experiments. Asc = ascaridole; BH‐SMP = submitochondrial particles from bovine heart; Car = carvacrol; Caryo = caryophyllene oxide; EO = essential oil; LtP‐Mit = mitochondrial crude fraction of Leishmania tarentolae; SD = standard deviation.