Fig. 2.

PRMT2 is required for GBM cell anchorage-dependent and -independent growth. a, b PRMT2 expression levels in U87 and T98G cells transduced with two different shRNA constructs targeting PRMT2 (shPRMT2-1 and shPRMT2-2) or scramble shRNA (shScr) were determined by RT-qPCR (a) and WB analysis (b). GAPDH serves as a loading control. The error bars in a denote SD, n = 2. c Anchorage-independent growth of U87 and T98G cells transduced with shScr or shPRMT2 was measured by colony formation in soft agar. Representative colonies were photographed after 4 weeks. Scale bar, 30 µm. d, e Cell growth curves of U87 and T98G cells (d), TPC1115 and TPC0411 cells (e) expressing the indicated shRNAs. MTS assay was employed at the specified time points to follow the rate of proliferation. Significance level was determined using Student’s two-sided t-tests. **p ≤ 0.01 and ***p ≤ 0.001. Error bars, ± SD, n = 3