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. 2018 Oct 31;9:4552. doi: 10.1038/s41467-018-06968-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — PRMT2 is responsible for the maintenance of H3R8me2a levels and target gene activation. a WB analysis of designated histone modification levels in U87 cells expressing shScr or shPRMT2. Histone 3 was used as loading control. b Representative IF images of U87 cells with PRMT2 and H3R8me2a antibody. Scale bar, 50 µm. c Piechart shows the proportion of total H3R8me2a peaks in the indicated regions. d Average profiles of H3R8me2a across a genomic window of ± 3000 bp surrounding the peak summit in shScr an shPRMT2. H3R8me2a peak number 13,078 in U87 shScr, 11,374 in U87 shPRMT2. e Comparison of PRMT2 target gene expression in U87 cells expressing shScr and shPRMT2. The PRMT2 target genes are divided into three subgroups (promoter, distal, gene body) according to the distribution of PRMT2-dependent H3R8me2a peaks. The bottom and the top of the boxes represent the first and third quartiles respectively. Lines within boxes indicate medians. Whiskers represent the most extreme data within 1.5 times of the interquartile range. Circles represent outliers. The P-values are calculated by Wilcoxon’s signed-rank test. f Genomic snapshots of RNA-seq analyses (top panel) and H3R8me2a ChIP-seq analyses (bottom panel) of representative genes in U87 shScr and shPRMT2 cells