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. 2018 Oct 31;8:16094. doi: 10.1038/s41598-018-34409-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Generally, miRNA expression levels in OVS are similar in Pro/Est and Met/Diest samples. (A) Eight miRNAs in the top 50 are present in different abundances in Pro/Est and Met/Diest samples. Six (12%) miRNAs are significantly more abundant in Met/Diest, and 2 (4%) miRNAs are significantly more abundant in Pro/Est, with a fold change ≥ 1.5. (^*P < 0.05, ^**P < 0.01). (B) MiRNA abundance is correlated between Pro/Est and Met/Diest samples. Data are from 272 miRNAs with abundances of at least 2TP2M in both biological replicates of either Pro/Est or Met/Diest samples. Data shown are from Biorep 2 (R² = 0.9655). Biorep 1 (not presented) shows a similar pattern with R² = 0.9486.