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. 2018 Oct 31;8:16094. doi: 10.1038/s41598-018-34409-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MiRNAs levels were significantly altered in sperm after OVS-sperm interaction, following co-incubation of sperm with estrus OVS. RT-qPCR reveals that for 3 miRNAs (miR-143, miR-34c, and miR-22) sperm show significantly elevated levels, reflecting oviductosomal delivery of miRNAs after co-incubation with OVS for 30 min only. Control sperm were co-incubated in the OVS vehicle, HTF. The fourth miRNA, let-7a -5p, showed significantly reduced levels in sperm after their co-incubation with OVS for 30 min and 3 h. RT-qPCR analyses were performed in triplicate, using pooled samples. Values are the average frequencies of miRNA for each sample. Significant levels are *P < 0.05, **P < 0.01, ***P < 0.001.