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. 2018 Oct 31;8:16100. doi: 10.1038/s41598-018-34301-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Decrease of CD133 levels by oxytetracycline. (A) CD133 expression level by oxytetracycline treatment in Huh7 and Hep3B cells. Cells were treated with indicated concentration of oxytetracycline for 48 hr, and CD133 expression levels were examined with FACS analysis. (B,C) Inhibition of protein synthesis by oxytetracycline. (B) HPG-labeled proteins were monitored by treatment of oxytetracycline in Huh7 and Hep3B cells. Nuclei were detected by staining with Hoechst 33342, and (C) HPG intensity was analyzed. (D,E) Degradation of CD133 protein by oxytetracycline. Huh7 cells (D) and Hep3B cells (E) were treated with oxytetracycline with indicated concentration and one day after 30 μg/ml cycloheximide was treated for 24 hr. After 48 hr from oxytetracycline treatment, cells were prepared for western blot. All images were obtained and analyzed with HCS system. Data are shown as the mean and standard deviation (SD) of triplicate experiments. Scale bar = 200 μm. *p < 0.05, **p < 0.005.