Figure 1.

RNAi-mediated targeting of s38 gene specifically in the follicle-cell population of D. melanogaster generates ovaries with severely reduced levels of s38 protein. (A) Confocal laser scanning microscopy (CLSM) images of representative stage-10 (left panel) and stage-14 (right panel) follicles derived from c355 > eGFP transgenic control flies, specifically expressing the eGFP reporter protein in their follicle cells. Number of follicles examined: 89. (B) The s38-transcript contents in s38-targeted (c355 > s38_RNAi) ovaries are presented with an -average- reduction of 30%, as compared to control (c355-GAL4/+) ones, via employment of an RT-PCR protocol and utilization of Actin as gene of reference. (C) Graphical presentation of Mascot score-based comparative measurements of relative protein abundance (presented in fold: “x”), in between s38-downregulated (Table S2) and control³⁸ ovaries. Scale Bar: 50 μm. *p < 0.05.